RNAi assay

From MIBBI

1. Types of silencing RNA reagent

2. Pretreatment protocol

3. Number of cells per well

4. Reagent

5. Instrument

5.1. Instrument name

5.2. Instrument manufacturer

5.3. Type of readout

5.4. Instrument settings

6. Delivery

6.1. Delivery type and protocol

6.1.1. Percentage of cell confluence (if applicable)

6.1.2. Complexing protocol

6.1.3. Complexing time

6.2. Delivery reagent

6.2.1. Delivery reagent type

6.2.2. Delivery reagent manufacturer

6.2.3. Delivery reagent name

6.2.4. Delivery reagent final concentration

6.3. Silencing reagent final concentration

7. Silencing RNA reagent¹

7.1. Silencing RNA reagent identifier

7.2. Silencing RNA reagent library description²

7.3. Silencing RNA reagent type

7.4. Number of different silencing RNA molecules per silencing RNA reagent

7.5. Silencing RNA reagent sequences³

7.6. Modifications to silencing RNA reagent⁴

7.7. Target gene ID or accession number⁵

7.8. Target gene official gene symbol⁷

7.9. Vector or plasmid reference⁸

8. Assay plate description

8.1. Assay plate manufacturer

8.2. Assay plate type

8.3. Assay plate identifier

8.4. Assay plate layout⁹

8.4.1. Position¹⁰

8.4.2. Reagent group¹¹

8.4.3. Silencing RNA reagent identifier

9. Assay plate

9.1. Time to assay point¹²

9.2. Time of exposure to silencing reagent

9.3. Media changes

9.3.1. Media composition

9.3.2. Time of media change

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Footnotes

¹ link to probe db

² Provider/Version number

³ If available

⁴ If applicable

⁵ NCBI/EMBL/DDBJ

⁶ accession.version

⁷ If available

⁸ If applicable

⁹ Provide information for each well

¹⁰ Row/Column

¹¹ Experimental/Control type

¹² From delivery of silencing RNA reagent

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Sources

MIARE

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Notes