Capillary electrophoresis

From MIBBI

1. General features

1.1. Date

1.2. Person or responsible role

1.3. Experiment type

1.4. Experiment aim

2. Sample

2.1. Sample name(s)

2.2. Sample solution

2.3. Sample preparation

3. Equipment

3.1. Capillary

3.1.1. Description of capillary

3.1.2. Capillary manufature or sources

3.1.3. Capillary dimensions

3.1.4. Conditioning of a new or regeneration of an exsisting capillary

3.2. Instrumentation and other equipment

3.2.1. Manufacturer

3.2.2. Model

3.2.3. Catalog number

3.3. Control and data collection software

3.3.1. Manufacturer

3.3.2. Name

3.3.3. Version

4. Run processes¹

4.1. Run descriptors

4.1.1. Temperature of capillary

4.1.2. Auxiliary data channels

4.1.3. Duration of data collection

4.2. Step descriptors

4.2.1. Step name

4.2.2. Step conditions

4.2.3. Pre‐conditioning, flush and wash and background electrolyte or ampholytes solutions.

4.3. Sample injection

4.3.1. Sample name

4.3.2. Volume of sample solution

4.3.3. Temperature of sample storage

4.3.4. Injection geometry

5. Detection

5.1. Type of detection

5.2. Detection specifics

5.3. Direct or indirect detection

5.4. Detector calibration

6. Electropherogram Data Processing

6.1. Integration protocol

6.2. Integration specifics

6.3. Peak efficiency

6.4. Mobility

6.5. Migration or Retention Time

6.6. Resolution

---

Footnotes

¹ A run is made up of various steps, each of which needs to be defined as specified (Step descriptors, Sample injection and Detection) as applicable; there are also parameters that should be specified across the whole run (Run descriptors).

---

Sources

MIAPE

---

Notes

This module identifies the minimum information required to report the use of capillary electrophoresis in a proteomics experiment, sufficient to support both the effective interpretation and assessment of the data and the potential recreation of the work that generated it.